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Recombinant Mouse tPAProtein,His Tag

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詳細(xì)介紹

 

性能參數(shù)

分子別名(Synonyms)

t-PA; tPA; Alteplase; Reteplase; PLAT;

表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)

Mouse tPA?Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser33-Gln559, which consists of two chains: chain A (Ser33-Arg308) and chain B (Ile309-Gln559).[Accession | P11214]

分子量大小(Molecular Weight)

The protein has a predicted MW of 60.57 kDa. It migrates to 13-15 kDa (chain A and chain B fragment), 30-34 kDa and 35-40 kDa based on SDS-PAGE result.

內(nèi)毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

純度(Purity)

> 95% as determined by SDS-PAGE

制劑(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

儲(chǔ)存條件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事項(xiàng)

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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