表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human AREG Protein is expressed from HEK293 with hFc tag at the N-terminal. It contains Ser101-Lys187.[Accession | P15514]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human AREG, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA. 

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.