分子別名（Synonyms）

Notch-3; Notch homolog 3;CADASIL;

表達區間及表達系統（Source）

Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala1378-Ser1640.[Accession | Q9UM47]

分子量大小（Molecular Weight）

The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on SDS-PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Human Notch 3, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.