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Recombinant Rat CD47 Protein,hFc Tag

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詳細介紹

性能參數(shù)

分子別名（Synonyms）	CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
表達區(qū)間及表達系統(tǒng)（Source）	Rat CD47 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Gln19-Lys140.[Accession \| P97829-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 40.40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.
內(nèi)毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
純度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Mouse SIRP alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA.
制劑（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重構(gòu)方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

儲存條件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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