表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Phe19-Arg238.[Uniprot | Q9NZQ7-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAG Eand HPLC

 活性（Activity）

ELISA Data:  Immobilized Human PD-1, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.93μg/ml determined by ELISA 

SPR Data: Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay 

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100

μg/ml is recommended. Dissolve the lyophilized protein in distilled water.