表達區間及表達系統（Source）

Recombinant Human MAGE-A4 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV]

分子量大小（Molecular Weight）

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human MAGE-A4 (HLA-A*02:01) Tetramer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.

SPR Data: Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human MAGE-A4 (HLA-A*02:01) Tetramer, His Tag with an affinity constant of 8.49pM as determined in SPR assay

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.